On November 5, 2024, the Department of Transportation (DOT) published a final rule in the Federal Register (89 FR 87792) that introduces significant updates to its regulated industry drug testing program. This new rule focuses on the implementation of oral fluid drug testing, an alternative to the traditional urine-based testing. These updates aim to enhance the flexibility, accuracy, and oversight of drug testing practices within the DOT-regulated industries. Below, we summarize the key changes and their implications for employers, service agents, and collectors.